[L-06]
BIOMASS OF MEDICINAL PLANTS IN NORTHERN MOUNTAIN REGION OF
REPUBLIC OF SRPSKA

Srđan LJUBOJEVIĆ, Jugoslav BRUJIĆ and Jovan TRAVAR
University of Banja Luka, Faculty of Forestry,
Av. Stepe Stepanovica 75, 78000 Banja Luka, Republic of Srpska, Bosnia and Herzegovina

INTRODUCTION

When we discuss the multiple use of forests, we frequently treat medicinal plants as inexhaustible natural resource, sometimes uncritically and without valid database. The aim of this research was to estimate the biomass of medicinal plants in Northern mountain region of Republic of Srpska; where under biomass we understand the useful live substance amount expressed in kg per ha of air-dried parts of herbs [6].


MATERIAL AND METHODS

As a representative of Northern Mountain region of Republic of Srpska, we took the Teslić forest management region. This region spreads over 48.000 ha of various forests and forest's lands within altitude 166 m and 1.383 m.

The medicinal herbs populations are in fact infinite in this area, so we used a sample in order to describe some of their characteristics and estimate their biomass. As a first step we divided above mentioned region in 12 less heterogeneous subsets or site types (Tab.1) on the basis of already known ecological-productive entities [4,7]. As the second step all stands in each ecological-productive entity have been numerated, and than some of them singled out, in a random manner as a sample, until at least 50 sample plots per hectare per each site type have been attained, with a few exceptions. As the third step, we spread the sample unit network at a 100 m distance in directions N-S and E-W. The sample unit was a circular surface of 10 m2 (r = 1.86 m) for low-growing plants and 100 m2 (r = 5.65 m) for trees and shrubs. On every sample unit we measured altitude, inclination and exposition and estimated the density of crown canopy so as to meet the site-specific relief and light conditions. The cover density ranged in decimal order from 1.0 (for very dense sites where no part of direct solar radiation reaches the ground) to 0.0 (for open sites, which receives the full amount of direct solar radiation). We counted individuals of each species of medicinal herbs. Finally, we collected and weighed their useful parts in fresh condition from each forest stand and than reduced their mass to air-dried condition using appropriate coefficient of dryness from literature [2. 5.]. In this way, data from 628 sample plots were gathered in 1999 [1,2,3]. Applying standard statistical tool [8], we arrived to the following results.


Table 1. Site types in the Teslić forest management region
Code
Description of site type
Ha
Number of
sample pl.
I
Beech forests and mixed beech and fir forests (with spruce) on various soils over silicate parent rocks
22.038
73
II
Beech forests and mixed beech and fir forests (with spruce) on various soils over serpentine parent rocks
5.433
54
III
Sessile oak forests and mixed beech and sessile oak forests 
on various soils over silicate parent rocks
2.198
40
IV
Pine and sessile oak forests on shallow soils over serpentine parent rocks
4.466
60
V
Pine and sessile oak forests on deep soils over serpentine parent r. 
6.828
52
VI
Young pine stands on deep soils over silicate parent rocks
1.430
52
VII
Young pine stands on various soils over serpentine parent rocks
2.495
81
VIII
Young coniferous stands (except pine) on various parent rocks, mainly silicate ones
123
51
IX
Forest glades and shrubbery in the range of beech and sessile oak forests
1.334
51
X
Strips under the power lines and by the forest roads
691
34
XI
Zone of river and stream banks
898
41
S
TOTAL FORESTS AND FOREST'S LAND
47.934
589
XII
Meadows and pastures within the range of forests
4.086
39

RESULTS AND DISCUSSION

Basic characteristics of study area

Basic characteristics of study area could be assumed through mean and categorized relief and canopy attributes (Tab.2). These results are largely in agreement with those reported by literature [7]


Table 2. Canopy and relief of site types according to sample plots
Atributes
Code of cite type
I
II
III
IV
V
VI
VII
VIII
IX
X
XI
XII
Canopy
(%)
1.0 - 0.7
93
74
62
23
40
52
51
65
-
3
17
-
0.6 - 0.3
4
24
38
65
54
44
32
31
4
9
17
3
0.2 - 0.0
3
2
-
12
6
4
17
4
96
88
66
97
Exposition
(%)
N, NE, NW
33
48
-
15
11
2
5
31
20
29
68
36
E, W, 0
38
28
35
32
31
19
16
37
57
35
19
38
S, SE, SW
29
24
65
53
58
79
79
32
23
36
13
26
Average altitude (m)
522
722
568
739
438
521
391
393
581
467
415
412
Average inclination (%)
31
30
27
30
24
23
24
22
18
25
6
15

Diversity

The eighty-six species of medicinal plants were found on the sample plots in this forest area which are, apart from being curative, at the same time attractive for market (previous demand for medicinal plants on ten market places all over the former Yugoslavia had been taken into consideration). They are: Achillea millefolium, Agrimonia eupatoria, Althaea officinalis, An- gelica silvestris, Arctium lappa, Artemisia spp., Atropa belladonna, Asarum europaeum, Asperula odorata, Bellis perennis, Betonica officinalis, Calamintha officinalis, Carlina aca-ulis, Centaurea cyanus, Cichorium intibus, Colchicum autumnale, Digitalis spp., Equisetum arvense, Erithraea centaurium, Eupatorium canabinum, Euphrasia officinallis, Fragaria spp., Filipendula spp., Geum urbanum, Glechoma hederacea, Hypericum perforatum, Lamium al- bum, Linaria vulgaris, Lycopus europaeus, Malva silvestris, Melittis melissophyllum, Mentha spp., Orchis spp., Origanum vulgare, Plantago spp., Pimpinela spp., Polygonatum officinale, Polypodium vulgare, Potentila tormentilla, Primula spp., Pulmonaria officinalis, Rubus ida-eus, Rubus spp. (R. hirtus, R. tomentosus, R. fruticosus et R. rusticans), Sanguisorba officina-lis, Sanicula europaea, Saponaria officinalis, Sedum spp., Symphytum officinale, Taraxacum officinale, Teucrium chamaedrys, Teucrium montanum, Thymus serpyllum, Thymus vulgaris, Trifolium spp., Tussilago farfara, Urtica dioica, Vaccinium myrtillus, Verbascum spp., Verbena officinalis, Veronica officinalis, Viola odorata, Viola tricolor, Betula pendula, Cerasus avium, Cerasus mahaleb, Cornus mas, Corylus spp., Crataegus spp., Hedera helix, Juglans spp., Juniperus communis, Malus silvestris, Ononis spinosa, Prunus spinosa, Pyrus communis, Rhamnus cathartica, Rhamnus frangula, Rosa spp (R. arvensis, R. canina, R. glutinosa, R. pendulina et R. spinosissima), Ruscus aculeatus, Salix alba, Sambucus nigra, Tilia grandifolia, Tilia parvifolia, Viscum album.

Biomass

Biomass of medicinal plants is a rather variable characteristic, which varies within and between species and site types.

Within a single sort of herb, biomass is mainly growing with increasing of accessible light intensity. For example, Fragariae herba is more than 25 times bigger in young coniferous stands with mean canopy of 0.7 than in beech and fir forests with mean canopy of 0.9, in spite of similar soils and parent rocks (Tab. 3 & 3.1).

If we compare total biomass between site types, we will come to the analogous conclusions. For instance, total biomass on strips under the power lines and by the forest roads amounted 328 kg per ha, while in high dense forests, such as first five site types (Tab.1) amounted less than 120 kg per ha. Meadows and pastures give the biggest biomas of about 690 kg per ha.

If we consider biomass of medicinal plants as a measure of habitat natural potentialities, the examined site types can be divided into three categories:

  1. Site types with very limited natural potentialities of medicinal herbs where total biomass is less or near 70 kg per ha,

  2. Site types with limited natural potentialities of medicinal herbs where total biomass varies between 100-200 kg per ha,

  3. Site types with unlimited natural potentialities of medicinal herbs where total

biomass exceeds 300 kg per ha.

Table 3. Biomass of medicinal plants in the Teslić forest management region
Code
Sort of drug
Code of site type (see table 1.)
I
II
III
IV
V
VI
Average biomass [kg/ha]
1 a
Millefolli flos
0.334
         
1 b
Millefolli herba
0.595
         
2
Agrimoniae herba  
0.061
0.041
   
0.149
3
Althaea herba            
4
Angelicae radix  
0.813
       
5
Bardanae radix            
6
Absinthii herba            
7 a
Belladonnae folium
0.117
0.078
       
7 b
Belladonnae radix
1.148
0.765
       
8
Asari herba cum rhizoma
0.771
4.730
0.446
0.005
   
9
Asperulae herba
0.915
0.363
0.265
0.010
   
10
Bellidis flos            
11
Betonicae herba    
0.139
0.020
0.458
0.030
12
Calaminthae herba
0.007
         
13
Carlinae radix            
14
Cyani flos            
15
Cychorii radix            
16
Colchici semen            
17
Digitalis folium          
2.350
18
Equiseti herba            
19
Centaurii herba            
20
Eupatorii radix  
0.231
       
21
Euphrasiae herba            
22 a
Fragariae fructus
0.069
1.066
0.288
0.666
0.232
0.314
22 b
Fragariae herba
0.120
1,850
0.459
1.060
0.369
0.500
23
Filipendulae radix  
0.204
       
24
Gei urbani rhizoma    
0.025
     
25
Glechomae herba            
26
Hyperici herba  
0.024
 
0.051
0.050
 
27
Lamii albi herba  
0.085
       
28
Linariae herba            
29
Lycopii herba            
30
Malvae folium            
31
Meliloti herba
0.001
0.018
0.003
0.018
0.023
 
32
Menthae herba
0.102
         
33
Salep tuber  
0.189
       
34
Origani herba            
35
Plantaginis folium  
0.249
0.887
     
36
Pimpinellae radix    
2.193
     
37
Polygonati rhizoma  
0.023
 
1.557
   
38
Polypodii rhizoma
0.393
         
39
Tormentillae rhizoma  
0.007
0.384
0.048
1.394
0.693
40 a
Primulae flos
0.012
0.082
0.166
0.039
0.051
0.011
40 b
Primulae radix
0.053
0.360
0.728
0.173
0.224
0.050
41
Pulmonariae folium  
0.191
0.043
     
42
Rubi idaei folium
0.060
8.530
4.10
8.268
5.877
0.085
43
Rubi fruticosi folium
32.379
43.632
27.936
47.097
58.714
47.202
44
Sanguisorbae rhizoma            
45
Saniculae herba
0.188
0.790
1.995
     
46
Saponariae radix            
47
Sedi acris herba            
48 a
Symphyti folium        
0.025
 
48 b
Symphyti radix        
0.147
 
49 a
Taraxaci herba
0.040
       
0.574
49 b
Taraxaci radix
0.157
       
0.245
50
Teucrii herba    
0.081
   
1.149
51
Teucrii montani herba      
0.979
0.645
0.097
52
Serpylli herba
0.195
13.146
0.220
28.759
12.634
8.253
53
Thymi herba            
54
Trifolii pratensi flos  
0.026
       
55
Farfarae folium
0.282
0.206
0.043
 
0.033
 
56 a
Urticae herba
0.629
 
0.040
     
56 b
Urticae radix
0.846
 
0.055
     
57 a
Myrtilli folium
1.209
9.519
0.578
1.360
2.506
1.569
57 b
Myrtilli herba
2.135
16.803
1.021
2.400
4.424
2.770
57 c
Myrtilli fructus
0.564
16.452
1.447
6.980
9.960
5.032
58
Verbasci flos  
0.018
 
0.079
0.022
0.040
59
Verbenae herba      
0.050
   
60
Veronicae herba
0.605
0.200
 
0.130
0.438
0.095
61 a
Violae flos    
0.001
0.002
0.008
0.004
61 b
Violae herba    
0.010
0.020
0.071
0.036
61 c
Violae radix    
0.039
0.079
0.281
0.140
62
Violae tricoloris herba
0.012
     
0.011
0.017
S
TOTAL BIOMASS
43.938
120.711
43.633
99.850
98.597
71.405
S
Number of low-growing plants
20
26
21
18
17
17
 
Species
Average number of plants per ha
63
Betula spp.
1.37
37
7.5
 
1.9
11.5
64
Cerasus avium
23.3
5.5
153.6
5
2
30.8
65
Cerasus mahaleb      
5
1.9
 
66
Cornus mas    
3.8
     
67
Corylus spp.
11
59
5
 
50
3.8
68
Crataegus spp.
4.1
 
25
10
 
15.4
69
Hedera helix
8.2
       
9.6
70
Juglans spp.            
71
Juniperus communis    
157.5
6.7
98.1
300
72
Malus sivlestris  
1.8
     
9.6
73
Ononis spinosa            
74
Prunus spinosa      
6.7
7.7
13.5
75
Pyrus communis
17.8
 
42.5
10
40.4
9.6
76
Rhamnus cathartica        
5.8
15.4
77
Rhamnus frangula
2.7
     
348.1
1.9
78
Rosa spp.
13.7
61.1
52.5
219
252
881
79
Ruscus acuelatus  
13
       
80
Salix alba            
81
Sambucus nigra
123.3
3.7
       
82
Sorbus aucuparia  
94.4
 
65
26.9
34.6
83
Sorbus torminalis  
3.7
7.5
66.7
169.2
 
84
Tilia grandifolia            
85
Tilia parvifolia  
1.8
       
86
Viscum album  
14.8
       
S
Number of trees and shrubs
9
11
9
9
12
13
S
Total number of species
29
37
30
27
29
30

Table 3.1. Horizontal continuation of table 3.
Code
Sort of drug
Code of site type (see table 1.)
VII
VIII
IX
X
XI
XII
Average biomass [kg/ha]
1 a
Millefolli flos
0.005
2.653
6.771
9.017
1.191
123.788
1 b
Millefolli herba
0.008
4.728
12.068
16.072
3.411
220.634
2
Agrimoniae herba  
0.543
0.087
0.293
0.406
0.284
3
Althaea herba      
1.737
   
4
Angelicae radix  
0.353
   
2.833
 
5
Bardanae radix        
1.005
 
6
Absinthii herba      
0.071
   
7 a
Belladonnae folium            
7 b
Belladonnae radix            
8
Asari herba cum rhizoma
0.144
0.245
 
0.053
2.331
 
9
Asperulae herba      
0.061
   
10
Bellidis flos    
0.003
0.008
 
0.465
11
Betonicae herba
1.252
6.859
1.356
 
0.329
0.020
12
Calaminthae herba      
0.572
0.047
0.287
13
Carlinae radix          
4.984
14
Cyani flos          
0.060
15
Cychorii radix    
0.050
0.080
0.060
0.070
16
Colchici semen
0.003
         
17
Digitalis folium  
1.683
 
20.163
   
18
Equiseti herba  
0.152
 
0.565
0.747
 
19
Centaurii herba
0.003
0.014
0.045
0.007
 
0.053
20
Eupatorii radix      
0.385
   
21
Euphrasiae herba    
0.214
   
10.94
22 a
Fragariae fructus
0.944
1.978
0.760
4.426
3.343
2.291
22 b
Fragariae herba
1.505
3.152
1.210
7.050
5.327
3.650
23
Filipendulae radix  
0.040
2.667
   
24.615
24
Gei urbani rhizoma      
1.016
   
25
Glechomae herba      
1.225
0.978
 
26
Hyperici herba
0.005
 
0.215
0.026
0.064
 
27
Lamii albi herba            
28
Linariae herba          
0.186
29
Lycopii herba      
0.334
0.783
 
30
Malvae folium      
0.131
 
0.175
31
Meliloti herba
0.028
         
32
Menthae herba  
0.916
 
2.286
 
4.484
33
Salep tuber          
0.128
34
Origani herba      
0.215
   
35
Plantaginis folium
0.139
0.359
13.913
13.015
2.321
64.967
36
Pimpinellae radix        
0.129
3.044
37
Polygonati rhizoma    
0.073
     
38
Polypodii rhizoma  
0.366
       
39
Tormentillae rhizoma
1.578
1.281
21.259
0.672
0.017
22.655
40 a
Primulae flos
0.047
0.026
 
0.113
0.108
 
40 b
Primulae radix
0.208
0.114
 
0.495
0.474
 
41
Pulmonariae folium  
0.013
   
0.063
 
42
Rubi idaei folium
3.062
2.258
2.343
3.388
4.969
0.226
43
Rubi fruticosi folium
59.767
59.271
33.202
188.604
63.506
15.228
44
Sanguisorbae rhizoma          
89.673
45
Saniculae herba      
0.040
0.168
 
46
Saponariae radix        
0.032
 
47
Sedi acris herba      
1.305
0.043
 
48 a
Symphyti folium            
48 b
Symphyti radix            
49 a
Taraxaci herba
0.142
   
0.629
0.684
13.909
49 b
Taraxaci radix
0.055
   
0.243
0.254
5.376
50
Teucrii herba
0.116
0.124
0.241
   
0.576
51
Teucrii montani herba
2.278
0.164
13.324
4.886
   
52
Serpylli herba
17.837
1.197
68.890
32.820
279.00
66.166
53
Thymi herba        
7.977
 
54
Trifolii pratensi flos    
0.014
 
0.103
 
55
Farfarae folium      
7.601
20.938
 
56 a
Urticae herba      
2.359
4.153
 
56 b
Urticae radix      
3.174
5.589
 
57 a
Myrtilli folium
2.556
1.648
0.943
0.036
   
57 b
Myrtilli herba
4.512
2.909
1.665
0.063
   
57 c
Myrtilli fructus
8.415
7.108
6.289
0.239
   
58
Verbasci flos
0.007
 
0.048
0.156
0.019
0.059
59
Verbenae herba    
0.074
 
0.231
 
60
Veronicae herba
0.095
0.241
0.519
1.431
0.067
6.231
61 a
Violae flos
0.006
 
0.039
0.017
0.014
0.086
61 b
Violae herba
0.046
0.007
0.046
0.141
0.119
0.720
61 c
Violae radix
0.180
0.027
1.296
0.556
0.471
2.843
62
Violae tricoloris herba
0.021
 
0.006
   
0.170
S
TOTAL BIOMASS
104.96
100.43
189.63
327.78
414.30
688.04
S
Number of low-growing plants
22
24
25
37
33
29
 
Species
Average number of plants per ha
63
Betula spp.
4.9
9.8
39.2
79.4
 
10.3
64
Cerasus avium
13.6
33.3
2
5.9
4.9
2.5
65
Cerasus mahaleb            
66
Cornus mas            
67
Corylus spp.  
147
9.8
32.3
48.8
 
68
Crataegus spp.
6.2
51
3.9
 
48.8
5.1
69
Hedera helix  
2
 
2.9
   
70
Juglans spp.  
3.8
       
71
Juniperus communis
179
158.8
249
11.8
21.9
125.6
72
Malus sivlestris  
9.6
22.2
7.8
64.7
33.3
73
Ononis spinosa  
1.96
       
74
Prunus spinosa  
9.9
3.9
   
5.1
75
Pyrus communis
166.7
35.3
33.3
21
7.3
51.3
76
Rhamnus cathartica
196.3
 
2.9
     
77
Rhamnus frangula
335.8
76.5
17.7
135.3
134.1
 
78
Rosa spp.
762
37.2
1192
6
17.1
197.4
79
Ruscus acuelatus            
80
Salix alba      
11.8
19.5
 
81
Sambucus nigra  
5,9
 
32.3
117.1
 
82
Sorbus aucuparia
58
 
82.3
 
9.8
 
83
Sorbus torminalis
135.8
5.9
5.9
5.9
2.4
2.6
84
Tilia grandifolia  
3.9
       
85
Tilia parvifolia            
86
Viscum album            
S
Number of trees and shrubs
10
16
13
12
12
9
S
Total number of species
32
40
38
49
45
38

LITERATURE
  1. Ljubojević S. (1984): Metodika izrade šumskoprivredne osnove za "Krivajsko" i "Kakanjsko" šumskoprivredno područje za uređajni period 1988-2002 godine - dio Sporedni šumski proizvodi. Šumarski fakultet u Sarajevu, 1-12.

  2. Ljubojević S., Alajbegović D., Beus V., Milanović S., Pinjuh M., Rončević S. (1987): Analiza sirovinske baze i osnove organizacije i razvoja djelatnosti prikupljanja i prerade ljekovitog bilja, šumskih plodova, gljiva i puževa u šumama SR BiH. SIPAD i Šumarski fakultet u Sarajevu, 1-191.

  3. Ljubojević S. (1998): Biomass of Medicinal plants in Beech forests on Calcisols in Central Dinaric region of Bosnia and Herzegovina. XVI World Congress of Soil Science, Montpellier, Proceedings, Symposium 33, 1-5.

  4. Stefanović V., Beus V., Burlica Č., Dizdarević H., Vukorep I. (1983): Ekološko-vegetacijska rejonizacija BiH Šumarski fakultet u Sarajevu, Posebna izdanja 17, 1-51.

  5. Tucakov J. (1984): Lečenje biljem. "Rad", Beograd, 1-717.

  6. Šumarska enciklopedija (1980): I tom, JLZ, Zagreb, 1-727.

  7. Šumskoprivredna osnova za "Teslićko" šumskoprivredno područje. Biro za istraživanje i projektovanje u šumarstvu, Banja Luka, (1998): 1-372.

  8. Statistic for Windows (1993): Release 4.5 A, StatSoft. Inc.

[L-06]